Thursday, August 22, 2019

Holger Heyn et al. Distinct DNA methylomes of newborns and Assignment

Holger Heyn et al. Distinct DNA methylomes of newborns and centenarians PNAS 2012 paper and answer the following questions - Assignment Example target points in the experiment not only because they contain methyl groups but also the researchers wanted to strengthen the study through CpG results, because they make the DNA strands which happen to play a crucial role in human aging. In relation to methylation, CpG regions tend to have a high content of methylomes hence were the most suitable for the study. 3. 12- 16 Whole Genome bisulphite sequencing of all the samples: newborn, centenarian and intermediate, was done to identify the methylation status. Also, a microarray of 450000 CpG methylation samples were included in the experiment. WGBS was hence performed for all the samples. For the newborn, fresh cord blood with permit from the parents was acquired for the experiment while for the old age his CD4 positive T cells were used. The intermediate sample was from a twenty six year old. All the three samples were from Caucasian males. 4. The methylation status in the three samples was different but formed a final curve in the results. The centenarian DNA had low methylomes compared to those in a newborns DNA. Moreover, there is very low correlation in methylation of the CpG positions that are near the DNA. The intermediate sample was between the two, meaning is was second in the level of methylation. The CpG that were densely methylated in centenarian sample were located in specific genomic regions such as the promoters and more were on Island promoters than the poor promoters. 5. Alternative methods used apart from WGBS included the determination of the DMRS which are the differentiated methylated regions in the DNA of the two main samples. This method involved checking the total length of identical CpGs in the two samples by counting those that have a continuous consistent flow of methylation status. Moreover, an identified map of CpG that showcases a microarray of the 450000 samples use. The results from the map were then compared to those from WGBS. 6. The six genes from WGBS that were identified as

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